File
Polymorphic microsatellite loci for tiger salamanders, Ambystoma tigrinum
Digital Document
Content type |
Content type
|
---|---|
Collection(s) |
Collection(s)
|
Resource Type |
Resource Type
|
Genre |
Genre
|
Peer Review Status |
Peer Review Status
Peer Reviewed
|
Origin Information |
|
---|
Persons |
Author (aut): Mech, Stephen G.
Author (aut): Storfer, Andrew
Author (aut): Ernst, J. A.
Author (aut): Reudink, Matthew W.
Author (aut): Maloney, S. C.
|
---|
Abstract |
Abstract
Microsatellites have been developed for few amphibian species. However, developing genetic markers for population genetic studies in amphibians is critical because amphibians are declining globally. The tiger salamander, Ambystoma tigrinum, is widespread throughout the United States and includes the endangered subspecies, A. t. stebbinsi. We present primers and amplification conditions for 10 polymorphic microsatellite loci that have produced successful results in three subspecies of
A. tigrinum. Number of alleles per locus ranged from one to 11 and heterozygosity ranged from 0 to 0.815 depending on the
subspecies and locus analysed. These markers should prove useful for future studies of genetic diversity and population subdivision. |
---|---|
Language |
Language
|
Publication Title |
Publication Title
|
---|---|
Publication Number |
Publication Number
Volume 3, Issue 1
|
Publication Identifier |
Publication Identifier
issn: 1471-8286
|
Publication Genre |
Publication Genre
|
DOI |
DOI
10.1046/j.1471-8286 .2003.00356.x
|
---|---|
Handle |
Handle
Handle placeholder
|
ISSN |
ISSN
1471-8278
|
Note |
|
---|
Use and Reproduction |
Use and Reproduction
author
|
---|
Keywords |
Keywords
tiger salamander
Ambystoma tigrinum
amphibian
microsatellite
|
---|---|
Subject Topic |
Subject Topic
|